Measurement

Part:BBa_J119096

Designed by: Jerrad Morton   Group: Eckdahl Lab   (2012-04-24)


pT7-RBS-RFP-pLac+tRNA CCACC+pLac+tRNA AGGAC+pLac+tRNA CUACU

Promoter for T7 RNAP polymerase drives RFP expression. Three frameshift suppression tRNAs produced: CCACC+AGGAC+CUACU.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 972
    Illegal BamHI site found at 1331
    Illegal BamHI site found at 1690
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 716
    Illegal AgeI site found at 1009
    Illegal AgeI site found at 1368
    Illegal AgeI site found at 1727
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None